LOCUS       sequence-135859-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6
            promoters).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroB...
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..25
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(187..435)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(245..264)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(415..435)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        complement(593..906)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(654..674)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc
       61 ggtagaattg gatccaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
      121 ctagccttat ttaaacttgc tatgctgttt ccagcatagc tcttaaacgt gtcttctcga
      181 agacccggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca
      241 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc
      301 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa
      361 attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat
      421 gggaaatagg ccctcttcct gcccgacctt ggacgcgtga gtgtgaaaca gttcggctaa
      481 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatttaaact
      541 tgctatgctg tttccagcat agctcttaaa ccgaagagcg atcgctcttc caaacaaggc
      601 ttttctccaa gggatattta tagtctcaaa acacacaatt actttacagt tagggtgagt
      661 ttccttttgt gctgtttttt aaaataataa tttagtattt gtatctctta tagaaatcca
      721 agcctatcat gtaaaatgta gctagtatta aaaagaacag attatctgtc ttttatcgca
      781 cattaagcct ctatagttac taggaaatat tatatgcaaa ttaaccgggg caggggagta
      841 gccgagcttc tcccacaagt ctgtgcgagg gggccggcgc gggcctagag atggcggcgt
      901 cggatcacgc gtagggcccg gtactaacca aactggatct ctgctgtccc tgtaataaac
      961 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     1021 tattatgtct actat
//
