LOCUS       sequence-135863-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Antisense Control Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL002
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(206..310)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     378..396
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(400..443)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        461..495
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     CDS             515..544
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             560..577
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(633..650)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      803..849
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(902..919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
       61 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
      121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
      181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
      241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
      301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
      361 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
      421 tagctttcgc taaggatgat ttctggaatt ctaaagatct ttgacagcta gctcagtcct
      481 aggtataata ctagtggatc tgaagcttgg gcccgaacaa aaactcatct cagaagagga
      541 tctgaatagc gccgtcgacc atcatcatca tcatcattga gtttaaacgg tctccagctt
      601 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
      661 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
      721 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
      781 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaactg gatccttact cgagtctaga ctgcaggctt
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1021 caaaaggcca gcaaa
//
