LOCUS       sequence-135871-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pbuE STAR Antisense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2191
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      245..833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     734..753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     987..1004
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
       61 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      121 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      181 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca
      841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      901 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      961 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgagga
//
