LOCUS       sequence-135877-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sense Control Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL001
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(190..236)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     389..406
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(462..479)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(495..524)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      121 gtgaaagttg gaacctctta cgtgcccgat caactgcagt ctagaaatta gttcaccgac
      181 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
      241 ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt
      301 cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt
      361 ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc
      421 tcatccgcca aaacagccaa gctggagacc gtttaaactc aatgatgatg atgatgatgg
      481 tcgacggcgc tattcagatc ctcttctgag atgagttttt gttcgggccc aagcttctca
      541 ttactcgagt taggatcttt agaattcagc cggaagcata aagtgtaaag cctctcggcg
      601 cgccatcgga agcttatcga tgataagctg tcaaacatga gaattacaac ttatatcgta
      661 tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt
      721 ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
      781 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
      841 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
      901 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
      961 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     1021 gcgcagcggt cggactgaac
//
