LOCUS       sequence-135881-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AD1 Sense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2801
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        186..220
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     CDS             join(334..336,337..1050)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             334..1047
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(364..392)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     987..1007
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
       61 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
      121 ataagcttcc gatggcgcgc cgagaggctt tacactttat gcttccggct gaattctaaa
      181 gatctttgac agctagctca gtcctaggta taatactagt agtttttaca gtgaattgtt
      241 ttaattagtt gtataaatgt tggagcagcg gggaatgtat acagttcatg tatatattcc
      301 ccgctttttt tttggatcta ggaggaagga tctatgagca aaggagaaga acttttcact
      361 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      421 cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt tatttgcact
      481 actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta tggtgttcaa
      541 tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc
      601 gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta caagacgcgt
      661 gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat
      721 tttaaagaag atggaaacat tcttggacac aaactcgagt acaactttaa ctcacacaat
      781 gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac
      841 aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc
      901 gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt cctttcgaaa
      961 gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt
     1021 acacatggca tggatgagct ctacaaataa ggatctgaag cttgggccct aacaaaa
//
