LOCUS       sequence-135886-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Anti-anti Sense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL1864
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..236
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(455..501)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     654..671
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(727..744)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(760..789)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(852..872)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
       61 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      121 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      181 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      241 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      301 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
      361 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactg cagtctagac
      421 tcgagtaagg atccagttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      481 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca
      541 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
      601 ccgcgctact gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa
      661 tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca gccaagctgg agaccgttta
      721 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
      781 tttttgttcg ggcccaagct tcagatcctt atttgtagag ctcatccatg ccatgtgtaa
      841 tcccagcagc agttacaaac tcaagaagga ccatgtggtc acgcttttcg ttgggatctt
      901 tcgaaaggac agattgtgtc gacaggtaat ggttgtctgg taaaaggaca gggccatcgc
      961 caattggagt attttgttga taatggtctg ctagttgaac ggaaccatct tcaacgttgt
     1021 ggcgaatttt gaagttagct ttgattccat tcttttgttt gtctgccgtg a
//
