LOCUS       sequence-135889-       10287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression of Ago2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 10287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pCW57.1 _3xHA-Ago2
FEATURES             Location/Qualifiers
     source          1..10287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             546..635
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAASYPYDVPDYA"
     promoter        3229..3739
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3658..3676
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3749..4345
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3749..3768)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4245..4265
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4346..4399
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4400..5017,5018..5023,5024..5059,5060..5062,5063..5098,
                     5099..5101,5102..5137,5138..5146)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5186..5206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5604..5615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5820..6053
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6125..6246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6162..6181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6216..6235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6286..6421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6421..6492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6493..6561,6562..7353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6711..6730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7524..8112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8013..8032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8266..8283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8400..8421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8436..8441,8442..8459,8460..8466)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8474..8490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8479..8501
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8498..8514
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8498..8514
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8533..8553
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8535..8553
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8581..8807
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8808..8988
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9035..9160
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9653..9886
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10071..10115
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcca
      541 ccatgtaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg
      601 cagcatccta tccatatgac gttccagatt acgctggatc ctactcggga gccggccccg
      661 ttcttgcttc tcctgctccg acaacatcac ccatcccagg atatgccttc aaacctccac
      721 ctcggccgga cttcggcacc accgggagaa caatcaaact acaggccaat ttctttgaaa
      781 tggacatccc caaaattgac atctatcact atgaattgga catcaaacct gagaaacgcc
      841 ctcggagagt gaacagggaa attgtggagc acatggtcca gcactttaaa actcagatct
      901 tcggggaccg gaagccagtg tttgatggaa ggaagaatct gtacacagca atgccccttc
      961 cgatcggcag ggacaaggtg gagctggagg tcacgctccc gggagaaggc aaagatcgca
     1021 tcttaaaggt atccatcaag tgggtgtcgt gcgtgagcct gcaggcgtta cacgatgcac
     1081 tttcggggcg gctgcccagc gtccccttcg agacgatcca ggccctggac gttgtcatga
     1141 ggcacttacc atccatgagg tacacccctg ttggccgttc cttcttcact gcatctgaag
     1201 gctgttccaa ccctctgggt gggggcagag aagtgtggtt tggcttccat cagtccgtcc
     1261 gaccttctct ttggaaaatg atgctgaata ttgatgtatc ggcaacagcg ttttacaagg
     1321 cacagccagt gatcgagttt gtttgtgaag ttttggattt taaaagtatt gaagaacaac
     1381 aaaaacctct gacagattcc caaagggtaa agtttaccaa agaaatcaaa ggtctaaagg
     1441 tggagataac gcactgtggt cagatgaaga ggaagtaccg tgtctgcaat gtgacccggc
     1501 ggcctgccag tcaccaaacg ttcccactgc agcaggagag tgggcagaca gtggagtgta
     1561 cagtggccca gtacttcaag gacaggcaca agctggttct gcgctacccc cacctcccgt
     1621 gtttacaagt cggacaggag cagaaacaca cctaccttcc tttggaggtc tgtaacatag
     1681 ttgctggaca gagatgtata aaaaaattaa cagacgatca gacctcaacc atgatcagag
     1741 caactgccag gtcagcacct gatcgccaag aggagatcag caaactgatg cgaagtgcaa
     1801 gtttcaatac agacccatat gttcgtgaat ttggaatcat ggtgaaagat gagatgacag
     1861 atgtgactgg gcgggttctg cagccgccct ccatcctcta cgggggcagg aacaaagcaa
     1921 ttgccacccc tgtccagggt gtgtgggaca tgcgaaacaa gcagttccac acgggcatcg
     1981 agatcaaggt gtgggccatc gcctgcttcg ctccccagcg ccagtgtaca gaagtccatc
     2041 tcaagtcctt cacagagcaa ctgaggaaga tctcgagaga tgctgggatg ccgatccagg
     2101 gtcagccctg cttctgtaaa tacgcacagg gtgcagacag tgtggagccc atgttccgac
     2161 acctgaagaa cacatacgct ggcctccagc tggtggtggt catcctgcct ggcaaaactc
     2221 ctgtgtatgc ggaagtcaag cgtgtgggag acacagtgct ggggatggcc acacagtgcg
     2281 tccagatgaa gaacgtgcag aggaccacgc cacagaccct atccaatctc tgcttaaaga
     2341 tcaatgtcaa actgggaggc gtcaacaaca tcctgctgcc acagggcagg cctccagtgt
     2401 tccagcaacc tgtcatcttc ctgggagccg atgtcaccca cccaccagct ggggatggga
     2461 agaaaccttc tattgctgcc gtcgtgggca gcatggacgc acaccccaac cgctactgtg
     2521 ccaccgtgcg cgtgcagcag caccgacagg agatcatcca ggacctggct gccatggtac
     2581 gagagttgct cattcagttc tacaagtcca cccgcttcaa gcccacccgc atcatcttct
     2641 accgcgatgg cgtctccgag ggccagttcc agcaggttct ccaccatgag ctcctggcca
     2701 tcagagaggc ctgcatcaag ctggagaagg actatcagcc aggaatcacg ttcatcgtgg
     2761 tgcagaagcg gcaccacaca cgcctcttct gcacagacaa aaatgagcgg gttgggaaga
     2821 gtgggaacat tcccgcaggc acaaccgtgg acacgaagat cacccacccc actgagtttg
     2881 acttctacct gtgcagtcac gccggcatcc aggggacagg ccgtccttcc cactaccacg
     2941 tgctttggga tgacaatcgt ttttcttctg atgagctgca gattctgacc taccagctgt
     3001 gtcacaccta cgtgcgctgc acacgctctg tgtcaatacc cgcgccagct tactatgctc
     3061 acctggtggc cttccgagcc aggtaccacc tggtggataa agaacatgac agcgctgaag
     3121 gaagccatac ctctgggcag agcaacgggc gagatcacca agcgctggcc aaggcggtcc
     3181 aggtccacca ggacacactg cgcaccatgt actttgcttg aggatcccgg ggttggggtt
     3241 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     3301 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     3361 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     3421 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     3481 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     3541 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     3601 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     3661 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     3721 caccgacctc tctccccagc aattcaccat gaccgagtac aagcccacgg tgcgcctcgc
     3781 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     3841 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     3901 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     3961 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4021 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4081 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4141 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4201 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4261 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4321 gtgcatgacc cgcaagcccg gtgccgaagg tagaggttct ctcctcactt gtggtgatgt
     4381 tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa acggagctct
     4441 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     4501 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     4561 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     4621 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     4681 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     4741 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     4801 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     4861 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     4921 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     4981 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     5041 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     5101 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa gtaaggatcg
     5161 atccaagata tcgtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5221 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5281 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5341 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5401 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5461 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5521 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5581 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5641 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5701 ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag
     5761 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5821 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6061 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6121 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6241 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     6301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6421 ctttcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6481 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6541 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6601 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6661 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6721 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6781 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6841 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6901 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6961 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7021 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7081 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7141 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7201 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7261 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7321 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7381 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7441 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7501 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7561 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7621 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7681 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7741 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7801 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7861 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7921 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7981 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8041 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8101 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8161 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8221 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8281 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8341 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     8401 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     8461 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8521 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     8581 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     8641 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     8701 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     8761 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     8821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8881 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8941 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     9001 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     9061 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     9121 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     9181 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     9241 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     9301 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     9361 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     9421 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     9481 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9541 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9601 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9661 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9721 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     9781 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     9841 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     9901 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     9961 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10021 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10081 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10141 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10201 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10261 agacccacct cccaaccccg aggggac
//
