LOCUS       sequence-135895-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-C1483F
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
      121 gctggaattc tgcagatatc catcacactg gcggccgcca tggattataa agatgatgat
      181 gatatgcttg gaaccggacc tgccgccgcc accaccgctg ccaccacatc tagcaatgtg
      241 agcgtcctgc agcagtttgc cagtggccta aagagccgga atgaggaaac cagggccaaa
      301 gccgccaagg agctccagca ctatgtcacc atggaactcc gagagatgag tcaagaggag
      361 tctactcgct tctatgacca actgaaccat cacatttttg aattggtttc cagctcagat
      421 gccaatgaga ggaaaggtgg catcttggcc atagctagcc tcataggagt ggaaggtggg
      481 aatgccaccc gaattggcag atttgccaac tatcttcgga acctcctccc ctccaatgac
      541 ccagttgtca tggaaatggc atccaaggcc attggccgtc ttgccatggc aggggacact
      601 tttaccgctg agtacgtgga atttgaggtg aagcgagccc tggaatggct gggtgctgac
      661 cgcaatgagg gccggagaca tgcagctgtc ctggttctcc gtgagctggc catcagcgtc
      721 cctaccttct tcttcc
//
