LOCUS       sequence-135898-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tRNA and synthetases for mammalian encoding of a 
            dinitrophenyl hapten, DnpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Ren W, Ji A, Wang MX, Ai HW
  TITLE     Expanding the Genetic Code for a Dinitrophenyl Hapten.
  JOURNAL   Chembiochem. 2015 Sep;16(14):2007-10. doi: 10.1002/cbic.201500204. 
            Epub 2015 Jul 28.
  PUBMED    26185102
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201500204"; journalName: "Chembiochem"; date: 
            "2015-09"; volume: "16"; issue: "14"; pages: "2007-10"
COMMENT     Sequence Label: pCMV-DnpK
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      304..313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacggaag
      301 cttgccacca tggataaaaa accattagat gttttaatat ctgcgaccgg gctctggatg
      361 tccaggactg gcacgctcca caaaatcaag caccatgagg tctcaagaag taaaatatac
      421 attgaaatgg cgtgtggaga ccatcttgtt gtgaataatt ccaggagttg tagaacagcc
      481 agagcattca gacatcataa gtacagaaaa acctgcaaac gatgtagggt ttcggacgag
      541 gatatcaata attttctcac aagatcaacc gaaagcaaaa acagtgtgaa agttagggta
      601 gtttctgctc caaaggtcaa aaaagctatg ccgaaatcag tttcaagggc tccgaagcct
      661 ctggaaaatt ctgtttctgc aaaggcatca acgaacacat ccagatctgt accttcgcct
      721 gcaaaatcaa ctccaaattc gtctgttccc
//
