LOCUS       sequence-135903-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses suppression tRNA and mCherry inserted with a Npu DnaE 
            intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Ren W, Ji A, Ai HW
  TITLE     Light activation of protein splicing with a photocaged fast intein.
  JOURNAL   J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. 
            Epub 2015 Feb 5.
  PUBMED    25647354
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja508597d";
            journalName: "J Am Chem Soc"; date: "2015-02-18- 18"; volume: "137";
            issue: "6"; pages: "2155-8"
COMMENT     Sequence Label: pMAH2-intein/mCherry
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(172..276)
                     /gene="bla"
                     /label=AmpR promoter
     tRNA            complement(join(315..361,362..364,365..398))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
     promoter        complement(407..621)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(467..486)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     tRNA            complement(join(652..698,699..701,702..735))
                     /product="amber suppressor variant of an E. coli leucine 
                     tRNA (Wu et al., 2004)"
                     /label=tRNA(CUA)
ORIGIN
        1 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
       61 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgac tcgacctcga
      301 ctcaaaaaaa ggactacccg gagcgggact tgaacccgca cagcgcgaac gccgagggat
      361 ttagaatccc ttgtgtctac cgattccacc atccgggcgg ggatctgtgg tctcatacag
      421 aacttataag attcccaaat ccaaagacat ttcacgttta tggtgatttc ccagaacaca
      481 tagcgacatg caaatattgc agggcgccac tcccctgtcc ctcacagcca tcttcctgcc
      541 agggcgcacg cgcgctgggt gttcccgcct agtgacactg ggcccgcgat tccttggagc
      601 gggttgatga cgtcagcgtt cgaattctcg acctcgactc aaaaaaagga ctacccggag
      661 cgggacttga acccgcacag cgcgaacgcc gagggattta gaatcccttg tgtctaccga
      721 ttccaccatc cgggcgggga tctgtggtct catacagaac ttataagatt cccaaatcca
      781 aagacatttc acgtttatgg
//
