LOCUS       sequence-135906-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of mCherry-TEV-S-tag CENP-C for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG123
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..732
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(170..188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     438..457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             778..798
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             811..855
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ggtggatcgc taccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc
       61 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      121 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      181 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      241 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      301 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      361 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      421 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      481 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
      541 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      601 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
      661 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      721 gagctgtaca agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag
      781 aatctttatt ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc
      841 cagcacatgg acagcgga
//
