LOCUS       sequence-135910-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Puromycin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-puro
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     292..312
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(370..786)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(386..402)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(418..440)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
       61 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      121 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
      181 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
      241 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
      301 gacacacatt ccacagggtc gaccactgtg ctggcgaatt cctacgtacc accacactgg
      361 gatccggccg gcgcctagag aaggagtgag ggctggataa agggaggatc gaggcggggt
      421 cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag gcggaggctt
      481 agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt cccaggtcac
      541 gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct tgatcttaac
      601 ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt taaaggttgg
      661 ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg actggttgtg
      721 agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag tggtaacagt
      781 ctggccctaa ttttcagaca aatacagaaa cacagtcaga cagagacaac acagaacgat
      841 gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc c
//
