LOCUS       sequence-135916-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS); hypothetical protein associated 
            with purSyQL cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK576
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..26)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     126..145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(825..841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(825..841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(838..860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    849..865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(873..879,880..897,898..903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    918..939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
       61 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      121 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaaggtac gtgtaaccgt
      181 catgctgaag gacggtgttc tggacccgca gggcaaggcc atcagccacg cattgcagac
      241 acttggtttt gaaggcgtgc acgatgtgcg cgtgggtaag gtgattgaac tggatctgcc
      301 gggtgccaaa gccgaggaag cccagaaaaa ggccgatgcc atggcgcgtg atctgctagc
      361 caacctggtg attgaagatt actctgtaga ggtggtggaa tgaagcgcac ccttcttata
      421 gcgctggcat tggcatgcgc ggcaccgggc ttggcccatg cccagcgcat tgcgcccatg
      481 aaggcaggta actttgcccg cctttgtacg cgcgccacta gtgctggcat ttgcgatgcc
      541 tatattggcg gcctgacgga tggggtctct ctttccaaaa tcaatgatcg gaacgagggg
      601 gatccttctg cgcctgctgg tttttgtgtt cccgctacag aaaccatgcc cgttatgcgc
      661 tccaaggtac ttacctggct gaaagcgcat tctgaaacac tggacaagcc ggttggtgaa
      721 agtgtttttg ccgccctgca cgcctcttat ccgtgcagtg tgaaaaaatg aaagcagcaa
      781 ttatcgtatt ccccggtacc ctcgagaagc ttggcgtaat catggtcata gctgtttcct
      841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1021 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
//
