LOCUS       sequence-135919-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK577
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..26)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     126..145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(457..473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(457..473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(470..492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    481..497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(505..511,512..529,530..535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    550..571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(688..705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(939..958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
       61 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      121 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaaggtac gtgtaaccgt
      181 catgctgaag gacggtgttc tggacccgca gggcaaggcc atcagccacg cattgcagac
      241 acttggtttt gaaggcgtgc acgatgtgcg cgtgggtaag gtgattgaac tggatctgcc
      301 gggtgccaaa gccgaggaag cccagaaaaa ggccgatgcc atggcgcgtg atctgctagc
      361 caacctggtg attgaagatt actctgtaga ggtggtggaa tgaaagcagc aattatcgta
      421 ttccccggta ccctcgagaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1021 ctttctccct tcggga
//
