LOCUS       sequence-135922-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Src inserted with a Npu DnaE intein (F1 construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Ren W, Ji A, Ai HW
  TITLE     Light activation of protein splicing with a photocaged fast intein.
  JOURNAL   J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. 
            Epub 2015 Feb 5.
  PUBMED    25647354
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja508597d";
            journalName: "J Am Chem Soc"; date: "2015-02-18- 18"; volume: "137";
            issue: "6"; pages: "2155-8"
COMMENT     Sequence Label: pcDNA3-intein/Src
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatgtcca aacacgccga tggcctgtgc caccgcctca ccaccgtgtg
      121 ccccacgtcc aagccgcaga ctcagggcct ggccaaggat gcctgggaga tccctcggga
      181 gtcgctgcgg ctggaggtca agctgggcca gggctagtta agctatgaaa cggaaatatt
      241 gacagtagaa tatggattat taccgattgg taaaattgta gaaaagcgca tcgaatgtac
      301 tgtttatagc gttgataata atggaaatat ttatacacaa cctgtagcac aatggcacga
      361 tcgcggagaa caagaggtgt ttgagtattg tttggaagat ggttcattga ttcgggcaac
      421 aaaagaccat aagtttatga ctgttgatgg tcaaatgttg ccaattgatg aaatatttga
      481 acgtgaattg gatttgatgc gggttgataa tttgccgaat atcaaaatag ccacacgtaa
      541 atatttaggc aaacaaaatg tctatgacat tggagttgag cgcgaccata attttgcact
      601 caaaaatggc ttcatagctt ctaattgctt tggcgaggtg tggatgggga cctggaacgg
      661 taccaccagg gtggccatca aaaccctgaa gcctggcacg atgtctccag aggccttcct
      721 gcaggaggcc caggtcatga agaagctgag g
//
