LOCUS       sequence-135926-       10760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Mayr C, Bartel DP
  TITLE     Widespread shortening of 3'UTRs by alternative cleavage and 
            polyadenylation activates oncogenes in cancer cells.
  JOURNAL   Cell. 2009 Aug 21. 138(4):673-84.
  PUBMED    19703394
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Aug 21. 138(4):673-84."
COMMENT     Sequence Label: pIS1 IMP-1 long-mut-let-7
FEATURES             Location/Qualifiers
     source          1..10760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     944..963
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1969
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8715..8836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8726..8745)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8780..8799
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8969..9073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9074..9142,9143..9934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9292..9311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10105..10693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10594..10613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
      781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
     1981 ctataccggt ccagcccctc cctgtccctt cgagtccagg acaacaacgg gcagaaatcg
     2041 agagtgtgct ctccccggca ggcctgagaa tgagtgggaa tccgggacac ctgggccggg
     2101 ctgtagatca ggtttgccca cttgattgag aaagatgttc cagtgaggaa ccctgatctc
     2161 tcagccccaa acacccaccc aattggccca acactgtctg cccctcgggg tgtcagaaat
     2221 tctagcgcaa ggcactttta aacgtggatt gtttaaagaa gctctccagg ccccaccaag
     2281 agggtggatc acacctcagt gggacgcaaa ctcaaatttc cttcaggttt taaaaacatg
     2341 cagagaggtg ttttaatcag ccttaaagga tggttcattt cttgacctta atgtttttcc
     2401 aatcttcttc cccctacttg ggtaattgac tcaaatacct ccatttacgg cctctttcta
     2461 tatttacact aattttttta tctttattgc taccagaaaa aaatgcgaac gaatgcattg
     2521 ctttgcttac agtattgact caagggaaaa gaactgtcag tatctgtaga ttaattccaa
     2581 tcactcccta accaataggt acaatatgga atgaagaaga ggggaaaatg gggagaaaga
     2641 tggttaaaat acataataat ccacgtttaa aaggagcgca cttgtggctg atctatgcca
     2701 gatcaccatc ttcaaattgg cacaactgaa atttccccac tctgttgggg cttccccacc
     2761 acattcatgt ccctctcccg tgtaggtttc acattatgtc caggtgcaca taggtggtat
     2821 tgaatgctca gcagggtagg ggctgaccac tgtccctgat tcccatcgtt ctcaggcgga
     2881 ttttatattt ttttaaagtc tattttaatg attggatatg agcactggga aggggacgct
     2941 aactcccctt gataaagtct cggttccatg gaggacttga gtggccccaa aggctgccac
     3001 ggtgccctca ccccagccca tgtgctccca taagggctgg ttcctagagg caggggttgt
     3061 ggggcactcc cagccacggc actgttacct tggtggtggg acttggaacc caaccctgag
     3121 ctcccgataa agctaaagtc catcatctgg caaattcagt aaattggaga gtacttgctt
     3181 ctgtttgtat ctgagaggaa tttttaactg acggcttctg tctccatgaa tcattatcag
     3241 catgatgaaa ggtgtgtcta aaaaacaatt cagaatacca gcagcattgt acagcaaggg
     3301 gtaaataagc ttaatttatt aatttaccag gcttaattaa gatcccatgg agtgtttagc
     3361 ccttgtggga gacagaagcc atcagttaaa tgaggttagg cctctcctcc taatatactg
     3421 attgacaatg catattagcc aggtaatgca ctttagctac cctggacaat gctatcaagt
     3481 gtgctgggaa gggaggaagg cctctctaca tatggaaaag cccatgcgtg gagttcccct
     3541 cctttcaaca ttgcaacaac agtaacaaca agacaaccgc aacatgtggg cgtagtcagg
     3601 caatgctgtg tgcgaagtaa actaacgcaa ggtatgaagt taacgcagca attattttcc
     3661 tttttgttcc ccccaacccc actcaaaaaa tttttttttg atttttgttt ttttgcagct
     3721 tgctgatatt ttatataaaa aagaaaagca aagcaaaaga gaagctgata gtcttgaata
     3781 ttttattttt ttaatgaaaa gaaaaaacaa gaaagttatg tttcataatt tcttacaaca
     3841 tgagccagta accctttagg aactctctat ggagaacagg cctggtggga aaggctttgg
     3901 gggctgcccc cttaggagga ggctagtgct aagagggaag gcccaggttt gagagagccc
     3961 agaggggcag agcccagagc cttgtttggc cctgatctct gacttctaga gccccagctg
     4021 ctggcggctg ctggaatatc ctacctgata ggattaaaag gcctagtgga gctgggggct
     4081 ctcagtggtt aaacaatgcc caacaaccaa ccagctggcc cttggtctcc tctctttcct
     4141 cctttggtta aagagcatct cagccagctt ttcccaccag tggtgctgtt gagatatttt
     4201 aaaatattgc ctccgtttta tcgaggagag aaataataac taaaaaatat accctttaaa
     4261 aaaacctata tttctctgtc taaaaatatg ggagctgaga ttccgttcgt ggaaaaaaga
     4321 caaggccacc ctctcgccct cagagaggtc cacctggttt gtcattgcaa tgcttttcat
     4381 tttttttttt tgttattgtt tcatttcagt tccgtcttgc tattcttcct aatctatatc
     4441 catagatcta aggggcaaac agatactagt taactgcccc cacctctgtc tccctgtctt
     4501 ctttagatcg gtctgattga ttttaaaagt ggacccaaac ttagggaatt cttgatttag
     4561 ggtggctggt ggcaaggagg ggcaggggat atggggacgt gactgggaca ggttcctgcc
     4621 ttatcatttt ctccctagga cattcccttg tagcccccag aattgtctgg cccaaattga
     4681 atagaagcag aaaaacattt agggataaca tcaggccagt agaattaagc ctctccacct
     4741 gtcccaacca taaaaagggt ctcccagctt tccatctctg gctctatatg ctttatccca
     4801 aaacaaagca gataacgttc agacgtcggc catttagtaa tttaaagcga atttccagca
     4861 gcaagcatgc tttgatatct ggttcagact atcatcagga agaaaaaaaa atcccacagt
     4921 acctgaaatg tgattgttgc agtgttcagt ttccttgggg gcctgctccc ttcacacctt
     4981 gagcccaagt ccttttccgt tggctgattc agctcccaga agagacgagg aagtgtgtgg
     5041 caagggactg gaaaacttca cttgcttgga ttaggcaagg ctccactcat tgttgatatt
     5101 tgcccagcag gaaaatcatg taagttatac caccagaaag caaaaggagc atggtttggt
     5161 ggttaaggtt tagtgggatg aaggacctgt cttggtgggc cgggccctct tgtgccccgt
     5221 aggctaggtc ttagggcaac tccttgccct cctgctcagc acctccattt ccccatcctt
     5281 ggtgagataa caagctatcg cgaaaagcac ttgggagatt tggatgattt gagaagagtg
     5341 acttaaaaaa aatgcttctg tgctctaaga tatatatgtg tgtgtgtgtg ctacatatat
     5401 atttttaaga aaggaccatc tctttaggat atatttttaa attctttgaa acacataacc
     5461 aaaatggttt gattcactga ctgactttga agctgcatct gccagttaca ccccaaatgg
     5521 ctttaatccc ctctcgggtc tggttgcctt ttgcagtttg ggttgtggac tcagctcctg
     5581 tgaggggtct ggttaggaga gagccatttt taaggacagg gagttttata gcccttttct
     5641 actttcctcc cctcctccca gtccttatca atcttttttc ctttttcctg accccctcct
     5701 tctggaggca gttgggagct atccttgttt atgcctcact attggcagaa aagaccccat
     5761 ttaaaaccca gagaacactg gagggggatg ctctagttgg ttctgtgtcc attttcctct
     5821 gtgccaaaga cagacagaca gaggctgaga gaggctgttc ctgaatcaaa gcaatagcca
     5881 gctttcgaca catacctggc tgtctgagga ggaaggcctc ctggaaactg ggagctaagg
     5941 gcgaggccct tcccttcaga ggctcctggg ggattagggt gtggtgtttg ccaagccaag
     6001 gggtagggag ccgagaaatt ggtctgtcgg ctcctggttg cactttgggg aaggagagga
     6061 agtttggggc tccaggtagc tccctgttgt gggactgctc tgtcccctgc ccctactgca
     6121 gagatagcac tgccgagttc ccttcaggcc tggcagacgg gcagtgagga ggggcctcag
     6181 ttagctctca agggtgcctt cccctcctcc caacccagac ataccctctg ccaaactggg
     6241 aaccagcagt gctagtaact aacgcacaga gccccagagg gcctgcttga gccttcttgc
     6301 tccacaggag aagctggtgc ctctaggcaa ccccttcctc ccacctctca tcaggggtgg
     6361 gggttctcct ttctttcccc tgaagtgttt atggggagat cctagtggct ttgccattca
     6421 aaccactcga ctgtttgcct gtttcttgaa aaccagtaga agggaaacag cacagcctgt
     6481 cacagtaatt gcaggaagat tgaagaaaaa tcctcatcaa tgccagggga cataaaagcc
     6541 atttcccttc caaatactcg acaatttaga tgcagaacat ttctctgtat tcagacttag
     6601 agtaacacca gctgaaaact gcagtttctt tcctttggat acataaggct tctctatcgg
     6661 ggtacgggac agggaggagg cctcatgtct gaagggggat ttaggggcga gagccccagc
     6721 cctgaccctc ggtcctgtgc accgctttgg ggcacagtct gatggcgcct ttgctggcgc
     6781 cttagtatgg ttgactccgg atggacaaaa gaaaaaaaat tttttttctt gaatgaaata
     6841 gcaggaagct cctcgggagc atgtgttttg attaaccgca ggtgatggat gctacgagta
     6901 taaatggatt aactaacgca atccttacag taagattgga actaagggca gggactcatg
     6961 cataagggta tgaatcccag ccaggacaag tgagttgagg cttgtgccac aaaaggtttg
     7021 tccttgggga acaggcaggc ctgccaggat cccccccata tcgattgggc tgggagggct
     7081 ggccatgagg tccccacttt ctgctttcct tgcccatgtg tcaccccttt ggcctccagc
     7141 ttgtccctct ctcactttct atagctttgt tggaccagat ggtgaggaaa ggaatggcct
     7201 cttcccttct agagggggct ggctggagtg agacctgggg cttggcctgg aacccaccac
     7261 acagccccaa agtcaggaag cctggggaaa ccagagctga gacctcttca acagggtttc
     7321 tttgagatcc tacacctcca ttgggccctt tttcagtctt caatgggggc ccagttggct
     7381 ctagaaggag aagaggtgaa gcaggatcct ttgccctggg ggagtctgag ggcgcggtcc
     7441 ttggactcat tcaggccgtc tttgtagttg ggggagttcc actgggcgat cccagcccct
     7501 ccccacccac cctctaatgg acctcctcat agaagcccca tttcactttt gttttatcta
     7561 cctcttagca aaacaataga taaattaggt agtggcagct ccacttgctt aggttagggg
     7621 gggaaaaaga tttctttttc caaaggaaaa aaatattacc ttgagaatac tttccaaaaa
     7681 ataaaattaa aaaaaaaaaa accaaaaaaa aaaatttttt tttaaaaggg agacattttc
     7741 cagtgaccac tggattgttt taatttccca agcttttttt tcccccataa ataagtttca
     7801 ctctttggcg attttcttca cttgtttaag ataacgtgct agctattcca acaggtaaca
     7861 gctttcacag tctgcccctg gcctgtctca ccccatcccc caccctattc ctgccagtga
     7921 gtccttcctg tgcttctctc ccttctcccc tcccagccag ctgacttcag tcacccctgt
     7981 cccccctccc ctgccaataa gctcccccag gaataaaggc tttgttttgg ggatgcttaa
     8041 atcttgactg gcacttcccg gctgtggggg ctggggagcc acttgtaaca tttctgtgca
     8101 gattttatgt tagccactgc tatgtaaaag cacgttcaaa atgaatttca gcagattatg
     8161 tgttaccata atgaataaac gtcctctatc accatttgga gtctcccttt tctccaggat
     8221 cttgatcctg gtccccaaaa ccagagtgaa tcaaaagagc ttcctcccct gaggcaaagt
     8281 ggatttgtaa gcagttctga aacatcactt actcagaaga gggaacgatg tattttgatg
     8341 agtgcaaatt gggaagagct ggaggcctac tgcttgggac agtttttttt tttttttttt
     8401 ttttaaatat gagtgctagc ttattctgta attgcggcaa ctttgaaaat tgtattttac
     8461 tggaaatctg ccagccatca ccacccgatt ttgattgtat ccttcctccc atcctttaat
     8521 ctgttcattg ctttggggga ggtggggcag ctggctcaca cgttggagtt tgttctttga
     8581 tggatgaacg aacactccag ttttctttcc cgtgaaggtt gtttcagcca caaaccactt
     8641 cattttgctg tttcaatttc aaaataaaag gaaacttata ttgaaagaca agcggccgct
     8701 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     8761 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     8821 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     8881 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     8941 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9001 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9061 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9121 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9181 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9241 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9301 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9361 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9421 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9481 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9541 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9601 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9661 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10741 ttttgctcac atggctcgac
//
