LOCUS       sequence-135927-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Src inserted with a Npu DnaE intein (F1 construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Ren W, Ji A, Ai HW
  TITLE     Light activation of protein splicing with a photocaged fast intein.
  JOURNAL   J Am Chem Soc. 2015 Feb 18;137(6):2155-8. doi: 10.1021/ja508597d. 
            Epub 2015 Feb 5.
  PUBMED    25647354
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja508597d";
            journalName: "J Am Chem Soc"; date: "2015-02-18- 18"; volume: "137";
            issue: "6"; pages: "2155-8"
COMMENT     Sequence Label: pcDNA3-intein/Src
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(49..753)
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     593..611
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atagggccct ctagatgcat gctcgagtta atggtggtga tggtgatgct tgtacagctc
       61 gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta
      121 gtcctcgttg tgggaaagga tgtccaactt gatgtcgacg ttgtaggcgc cgggcagctg
      181 cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt
      241 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
      301 agtggaggcc tcccagccca tggtacgaca ctgcattacg gggccgtcgg aggggaagtt
      361 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt
      421 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccaattga agccctcggg
      481 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc
      541 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac
      601 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
      661 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat
      721 gatggccatg ttatcctcct cgcccttgct cacgaattcg aggttctccc
//
