LOCUS       sequence-135930-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  serves as template for PCR-mediated fusion of U6-promoter with 
            sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB17
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        262..280
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acaaaagctc tgaagtcttc ttcatttaaa ttcttagatg atacttcatc tggaaaattg
       61 tcccaattag tagcatcacg ctgtgagtaa gttctaaacc atttttttat tgttgtatta
      121 tctctaatct tactactcga tgagttttcg gtattatctc tatttttaac ttggagcagg
      181 ttccattcat tgtttttttc atcatagtga ataaaatcaa ctgctttaac acttgtgcct
      241 gaacaccata tccatccggc gtaatacgac tcactatagg gagagcggcc gccagatctt
      301 ccggatggct cgagtttttc agcaagatgc tcacctgtga ttgctcctac tcaaatacaa
      361 aaacatcaaa ttttctgtca ataaagcata tttatttata tttattttac aggaaagaat
      421 tccttttaaa gtgtatttta acctataatg aaaaacgatt aaaaaaaata cataaaataa
      481 ttcgaaaatt tttgaatagc ccaggttgat aaaaattcat ttcatacgtt ttataactta
      541 tgcccctaag tattttttga ccatagtgtt tcaattctac attaatttta cagagtagaa
      601 tgaaacgcca cctactcagc caagaggcga aaaggttagc tcgccaagca gagagggcgc
      661 cagtgctcac tactttttat aattctcaac ttctttttcc agactcagtt cgtatatata
      721 gacctatttt caatttaacg tcgtttaata cgactcacta tatctttcta gaagatctcc
      781 tacaatattc tcagctgcca tggaaaatcg atgttcttct t
//
