LOCUS       sequence-135952-        5706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhodopsin promoter from chicken (Gallus gallus) gDNA 
            chr12:19,499,638–19,501,514 in galGal4 driving DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Enright JM, Lawrence KA, Hadzic T, Corbo JC
  TITLE     Transcriptome profiling of developing photoreceptor subtypes reveals
            candidate genes involved in avian photoreceptor diversification.
  JOURNAL   J Comp Neurol. 2015 Mar 1;523(4):649-68. doi: 10.1002/cne.23702. 
            Epub 2014 Dec 1.
  PUBMED    25349106
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.23702";
            journalName: "J Comp Neurol"; date: "2015-03-1- 1"; volume: "523"; 
            issue: "4"; pages: "649-68"
COMMENT     Sequence Label: Gg 1.8kb rhodopsin DsRed
FEATURES             Location/Qualifiers
     source          1..5706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1962..1971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1968..2645
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2148..2168)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2560..2583
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2730..2749)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2795..2850
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2849..2868)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3209..3225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3209..3225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3222..3244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3233..3249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3257..3263,3264..3281,3282..3287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3302..3323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3382..3577
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3428..3563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3583..3717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3620..3639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3674..3693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3785..3802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3956..4544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4036..4055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4715..5506,5507..5575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5338..5357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5576..5680)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacgata tctctagaga ttcgcccttg gtaccgctcg agggacttag taccggcaga
       61 ggatgtgtga ggaggtggca tttgcacacc aacagcagga ggactttggt ggctgtgaag
      121 gcaatagagg cccacctttg gttatgggaa gccaggcttt cagtggtaac aatgcttctg
      181 tgagatgttc cccacataga ggaagagttt ctctgcttgg ccttctcttt gaagagaaca
      241 caaagctttc tgctttctcc cttcgaactg ttttgcgtgc actgagctaa cctgcaggga
      301 acactggtaa aaacacggca ggaactgaag ctggttaatt aaggccaagc tgacacaagc
      361 ccttgctcat ctctcaactg cgagctccag gcctggcgca tccccctctg tgctgtatgg
      421 ggcactcagg tacccctcct ctgctcttgg ctgcattgac gtagcgagaa aagtgataaa
      481 cggaggatgc aaaaagggga agagatgggg gcaaatagct ttggaagctg aaggactgat
      541 cacagtggtg tgaactataa agaataacct tgtccagcaa agagaccctt tgtggtaggc
      601 ttcactttga aaagttaaaa ccagagaaca tctaacagct gctggctagc atgaagaatg
      661 ttcaaaacaa caccgggcac atcaagcagg gagacagatc taacgacagc ttcccctgca
      721 gctctaatgc ccatctccat ggaactcatc acttttgcag ggtttcaagg agactgaaga
      781 tgtctgtcat cagagtaaga ggtcaaatgt tggcttcagt tcagaacttg tgccttgtcc
      841 acaacccttc tctggatcaa ggcagaacga tatctgtcca agacagatgg acagatggag
      901 gttatttttt aggtgctgcc gatgtagctg ctgtgatgca aactgtgtat gaagacaaga
      961 acagaggagg tgatgcaact ggaagcctgc tagcaggatc agtgcttcac tgtgctgttc
     1021 aaaccttacc agacaaattt tgcagcacaa aaagcaggtg gctgagagcg catagaagaa
     1081 gtgctgctga tggccatgct gccaagcaca gcctttcatc agcctacccc tgaaaatggc
     1141 agctgtgacg ggacaggctg ctgtcacagc cctgcagtcc tgctgtcaga gcaggcccct
     1201 gctatggagg gactgcacca agcacgtcca gcccagccgc tgcagtgccc agacccatgg
     1261 cctgggggtt ctgaaccaca aaattcattc cagtttgctt tggtcaagca caaccacagg
     1321 aaggatcctg tgtgctttgt ctttctctga cgctctttgc ccgttggcct ggcagtaacg
     1381 tgtcccattt cccatatccc tctgtgggta aactgaggca ttttgaactg atgggcgctg
     1441 cagcctgtga tgggctgatg gggcaccctg tggggcacgg ggcggtgggc gtccagcccg
     1501 ggctgcggcg ggcaatgagt gctttgggcc caggcagcgg gcgctcagcc ctggggcagc
     1561 agcacagtgt gccagggagc gctgtgtgca ggtggggctg ccgtccggtt tgggctccca
     1621 cacaagcagt gcagagcttc ctgtctgcgt atgaccatga accgtggtct ccaacaatga
     1681 cagtgtgggc cagaaacagg tgtcacctta atacccaccc agaaaagcca attacccctg
     1741 gatgattatg attaataact ctctaagccc taatcgcgct gacaccgatg cccggggctt
     1801 actgcagggg gcactttata agggacgccc tacagctgct tctctgcggg gcgacagcag
     1861 aggctgggca gcggtgcgag cccaactgca gggtgattcc cccatacaaa aaccgacgct
     1921 gagaattccc gacggtaccg cgggcccggg atccaccggt cgccaccatg gcctcctccg
     1981 agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc
     2041 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga
     2101 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     2161 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc
     2221 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2281 cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca
     2341 tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg
     2401 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc
     2461 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga
     2521 agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc acctcccaca
     2581 acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc
     2641 tgtagcggcc gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     2701 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     2761 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     2821 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     2881 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg
     2941 ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     3001 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     3061 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     3121 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
     3181 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3241 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3301 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3361 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3421 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3481 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3541 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     3601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     3721 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3781 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3841 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3901 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3961 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4021 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4081 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4141 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4201 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4261 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4321 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4381 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4441 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4501 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4561 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4621 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4681 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4741 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4801 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4861 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4921 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4981 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5041 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5101 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5161 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5221 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5281 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5341 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5401 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5461 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5521 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5581 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5701 cacctg
//
