LOCUS       sequence-135955-        5750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhodopsin promoter from chicken (Gallus gallus) gDNA 
            chr12:19,499,638–19,501,514 in galGal4 driving GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Enright JM, Lawrence KA, Hadzic T, Corbo JC
  TITLE     Transcriptome profiling of developing photoreceptor subtypes reveals
            candidate genes involved in avian photoreceptor diversification.
  JOURNAL   J Comp Neurol. 2015 Mar 1;523(4):649-68. doi: 10.1002/cne.23702. 
            Epub 2014 Dec 1.
  PUBMED    25349106
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.23702";
            journalName: "J Comp Neurol"; date: "2015-03-1- 1"; volume: "523"; 
            issue: "4"; pages: "649-68"
COMMENT     Sequence Label: Gg 1.8kb rhodopsin GFP
FEATURES             Location/Qualifiers
     source          1..5750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1962..1971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1968..1970,1971..1973,1974..2687)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1968..1970,1971..1973,1974..2684)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2013..2034)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2274..2293)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2621..2642
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2774..2793)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2839..2894
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2893..2912)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3253..3269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3253..3269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3266..3288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3277..3293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3301..3307,3308..3325,3326..3331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3346..3367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3426..3621
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3472..3607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3627..3761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3664..3683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3718..3737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3829..3846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4000..4588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4080..4099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4759..5550,5551..5619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5382..5401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5620..5724)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacgata tctctagaga ttcgcccttg gtaccgctcg agggacttag taccggcaga
       61 ggatgtgtga ggaggtggca tttgcacacc aacagcagga ggactttggt ggctgtgaag
      121 gcaatagagg cccacctttg gttatgggaa gccaggcttt cagtggtaac aatgcttctg
      181 tgagatgttc cccacataga ggaagagttt ctctgcttgg ccttctcttt gaagagaaca
      241 caaagctttc tgctttctcc cttcgaactg ttttgcgtgc actgagctaa cctgcaggga
      301 acactggtaa aaacacggca ggaactgaag ctggttaatt aaggccaagc tgacacaagc
      361 ccttgctcat ctctcaactg cgagctccag gcctggcgca tccccctctg tgctgtatgg
      421 ggcactcagg tacccctcct ctgctcttgg ctgcattgac gtagcgagaa aagtgataaa
      481 cggaggatgc aaaaagggga agagatgggg gcaaatagct ttggaagctg aaggactgat
      541 cacagtggtg tgaactataa agaataacct tgtccagcaa agagaccctt tgtggtaggc
      601 ttcactttga aaagttaaaa ccagagaaca tctaacagct gctggctagc atgaagaatg
      661 ttcaaaacaa caccgggcac atcaagcagg gagacagatc taacgacagc ttcccctgca
      721 gctctaatgc ccatctccat ggaactcatc acttttgcag ggtttcaagg agactgaaga
      781 tgtctgtcat cagagtaaga ggtcaaatgt tggcttcagt tcagaacttg tgccttgtcc
      841 acaacccttc tctggatcaa ggcagaacga tatctgtcca agacagatgg acagatggag
      901 gttatttttt aggtgctgcc gatgtagctg ctgtgatgca aactgtgtat gaagacaaga
      961 acagaggagg tgatgcaact ggaagcctgc tagcaggatc agtgcttcac tgtgctgttc
     1021 aaaccttacc agacaaattt tgcagcacaa aaagcaggtg gctgagagcg catagaagaa
     1081 gtgctgctga tggccatgct gccaagcaca gcctttcatc agcctacccc tgaaaatggc
     1141 agctgtgacg ggacaggctg ctgtcacagc cctgcagtcc tgctgtcaga gcaggcccct
     1201 gctatggagg gactgcacca agcacgtcca gcccagccgc tgcagtgccc agacccatgg
     1261 cctgggggtt ctgaaccaca aaattcattc ctgtttgctt tggtcaagca caaccacagg
     1321 aaggatcctg tgtgctttgt ctttctctga cgctctttgc ccgttggcct ggcagtaacg
     1381 tgtcccattt cccatatccc tctgtgggta aactgaggca ttttgaactg atgggcgctg
     1441 cagcctgtga tgggctgatg gggcaccctg tggggcacgg ggcggtgggc gtccagcccg
     1501 ggctgcggcg ggcaatgagt gctttgggcc caggcagcgg gcgctcagcc ctggggcagc
     1561 agcacagtgt gccagggagc gctgtgtgca ggtggggctg ccgtccggtt tgggctccca
     1621 cacaagcagt gcagagcttc ctgtctgcgt atgaccatga accgtggtct ccaacaatga
     1681 cagtgtgggc cagaaacagg tgtcacctta atacccaccc agaaaagcca attacccctg
     1741 gatgattatg attaataact ctctaagccc taatcgcgct gacaccgatg cccggggctt
     1801 actgcagggg gcactttata agggacgccc tacagctgct tctctgcggg gcgacagcag
     1861 aggctgggca gcggtgcgag cccaactgca gggtgattcc cccatacaaa aaccgacgct
     1921 gagaattccc gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg
     1981 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2041 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2101 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2161 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2221 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2281 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2341 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2401 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2461 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2521 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2581 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2641 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcactc
     2701 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     2761 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     2821 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     2881 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     2941 agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg
     3001 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     3061 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     3121 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     3181 ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca gcccaagctt
     3241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     3421 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3481 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3541 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3601 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     3661 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3721 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     3781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     4201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4441 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4801 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5041 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5221 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5341 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5581 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5641 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5701 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
