LOCUS       sequence-135964-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET28a modified to contain TEV protease fusion tag instead of T7 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Kappock
  TITLE     Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK635
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             108..140
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             190..207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(260..278)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      274..321
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      358..813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(445..464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     655..676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggc acaccatcat caccatcatt
       61 caagcggcgt ggatctgggc accgaaaatc tgtactttca gtctcatatg gctagcatga
      121 ctggtggaca gcaaatgggt cgcggatccg aattcgagct ccgtcgacaa gcttgcggcc
      181 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      241 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      301 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      361 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      421 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      481 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      541 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      601 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
      661 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      721 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      781 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
      841 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta
      901 attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
      961 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactc
//
