LOCUS       sequence-135971-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit with N-terminal His6 tag (H6AaPurS); 
            hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK522
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(465..482)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(731..748)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(758..775)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 ctcgagggta ccggggaata cgataattgc tgctttcatt ttttcacact gcacggataa
      121 gaggcgtgca gggcggcaaa aacactttca ccaaccggct tgtccagtgt ttcagaatgc
      181 gctttcagcc aggtaagtac cttggagcgc ataacgggca tggtttctgt agcgggaaca
      241 caaaaaccag caggcgcaga aggatccccc tcgttccgat cattgatttt ggaaagagag
      301 accccatccg tcaggccgcc aatataggca tcgcaaatgc cagcactagt ggcgcgcgta
      361 caaaggcggg caaagttacc tgccttcatg ggcgcaatgc gctgggcatg ggccaagccc
      421 ggtgccgcgc atgccaatgc cagcgctata agaagggtgc gcttgtggtg gtggtggtgg
      481 tggctcattc caccacctct acagagtaat cttcaatcac caggttggct agcagatcac
      541 gcgccatggc atcggccttt ttctgggctt cctcggcttt ggcacccggc agatccagtt
      601 caatcacctt acccacgcgc acatcgtgca cgccttcaaa accaagtgtc tgcaatgcgt
      661 ggctgatggc cttgccctgc gggtccagaa caccgtcctt cagcatgacg gttacacgta
      721 ccttcatatg gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc
      781 tg
//
