LOCUS       sequence-135975-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK532
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             868..885
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(938..956)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      952..999
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaaagcag caattatcgt attccccggc
      121 acaaaccggg agcgcgatat ggccattgcc ctcaagcgcg ttacagggca ggccccgcgc
      181 atgatctggc accatgaaac agatctggga gataccgatc tagtcgtgct gccaggtggt
      241 ttcagctatg gcgattatct gcgctgtggt gccatggcag cgcattctcc catcatggcg
      301 gctatcaaag cctttgccca aaagggtggc catattttgg gtgtgtgcaa cggcttccag
      361 attttaacgg aaacagggct tctgcccggt gctctgttgc gcaacgccaa cctgcgcttt
      421 ctctctcagg attgctacct gaaggtggaa gcagagaaca cacctttcac tcacaagtgg
      481 aagaagggtg atgttttccg cacccctctg gcccatggtg atggcaatta cacggcagat
      541 atcgccacgc tggaacggct tgaaggtgaa ggtcttgtgg ctttccacta tgccgatgcc
      601 aaaggcaatg tgcaggctga tgatgccatc agcaacccca atggcagcat caacagcatt
      661 gcgggtattc tgagcgaaaa caaacgcatt tgcggcatga tgcctcatcc agaagatctg
      721 gttgatcctc tcatgggtgg ggaagatggt ctgccattgt tccaaggtct ggtggaggct
      781 gtagtccggt gaataagcct gtaaccgttg atgaatccct tgcccgtgaa ttcgagctcc
      841 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      901 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      961 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     1021 ggattggcga
//
