LOCUS       sequence-135976-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK532
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(820..837)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(847..864)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             883..905
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    920..944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(944..963)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(945..963)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattca cgggcaaggg attcatcaac ggttacaggc ttattcaccg
      121 gactacagcc tccaccagac cttggaacaa tggcagacca tcttccccac ccatgagagg
      181 atcaaccaga tcttctggat gaggcatcat gccgcaaatg cgtttgtttt cgctcagaat
      241 acccgcaatg ctgttgatgc tgccattggg gttgctgatg gcatcatcag cctgcacatt
      301 gcctttggca tcggcatagt ggaaagccac aagaccttca ccttcaagcc gttccagcgt
      361 ggcgatatct gccgtgtaat tgccatcacc atgggccaga ggggtgcgga aaacatcacc
      421 cttcttccac ttgtgagtga aaggtgtgtt ctctgcttcc accttcaggt agcaatcctg
      481 agagagaaag cgcaggttgg cgttgcgcaa cagagcaccg ggcagaagcc ctgtttccgt
      541 taaaatctgg aagccgttgc acacacccaa aatatggcca cccttttggg caaaggcttt
      601 gatagccgcc atgatgggag aatgcgctgc catggcacca cagcgcagat aatcgccata
      661 gctgaaacca cctggcagca cgactagatc ggtatctccc agatctgttt catggtgcca
      721 gatcatgcgc ggggcctgcc ctgtaacgcg cttgagggca atggccatat cgcgctcccg
      781 gtttgtgccg gggaatacga taattgctgc tttcatatgg ctgccgcgcg gcaccaggcc
      841 gctgctgtga tgatgatgat gatggctgct gcccatggta tatctccttc ttaaagttaa
      901 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta
      961 ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgc
//
