LOCUS       sequence-135977-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit, N-term His6 tag; glutaminase subunit; 
            large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK618
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgaa ggtacgtgta accgtcatgc
      121 tgaaggacgg tgttctggac ccgcagggca aggccatcag ccacgcattg cagacacttg
      181 gttttgaagg cgtgcacgat gtgcgcgtgg gtaaggtgat tgaactggat ctgccgggtg
      241 ccaaagccga ggaagcccag aaaaaggccg atgccatggc gcgtgatctg ctagccaacc
      301 tggtgattga agattactct gtagaggtgg tggaatgaaa gcagcaatta tcgtattccc
      361 cggtaccaac cgggagcgcg atatggccat tgccctcaag cgcgttacag ggcaggcccc
      421 gcgcatgatc tggcaccatg aaacagatct gggagatacc gatctagtcg tgctgccagg
      481 tggtttcagc tatggcgatt atctgcgctg tggtgccatg gcagcgcatt ctcccatcat
      541 ggcggctatc aaagcctttg cccaaaaggg tggccatatt ttgggtgtgt gcaacggctt
      601 ccagatttta acggaaacag ggcttctgcc cggtgctctg ttgcgcaacg ccaacctgcg
      661 ctttctctct caggattgct acctgaaggt ggaagcagag aacacacctt tcactcacaa
      721 gtggaagaag ggtgatgttt tccgcacccc tctggcccat ggtgatggca attacacggc
      781 agatatcgcc acgctggaac ggcttgaagg tgaaggtctt gtggctttcc actatgccga
      841 tgccaaaggc aatgtgcagg ctgatgatgc catcagcaac cccaatggca gcatcaacag
      901 cattgcgggt attctgagcg aaaacaaacg catttgcggc atgatgcctc atccagaaga
      961 tctggttgat cctctcatgg gtggggaaga tggtctgcca ttgttccaag gtctggtgga
     1021 ggctgtagtc cggtgaataa gcctgtaacc gttgatgaat cc
//
