LOCUS       sequence-135979-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit, N-term His-tag; hypothet. protein; 
            glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, 
            AaPurL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK619
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgaagg tacgtgtaac cgtcatgctg
      121 aaggacggtg ttctggaccc gcagggcaag gccatcagcc acgcattgca gacacttggt
      181 tttgaaggcg tgcacgatgt gcgcgtgggt aaggtgattg aactggatct gccgggtgcc
      241 aaagccgagg aagcccagaa aaaggccgat gccatggcgc gtgatctgct agccaacctg
      301 gtgattgaag attactctgt agaggtggtg gaatgaagcg cacccttctt atagcgctgg
      361 cattggcatg cgcggcaccg ggcttggccc atgcccagcg cattgcgccc atgaaggcag
      421 gtaactttgc ccgcctttgt acgcgcgcca ctagtgctgg catttgcgat gcctatattg
      481 gcggcctgac ggatggggtc tctctttcca aaatcaatga tcggaacgag ggggatcctt
      541 ctgcgcctgc tggtttttgt gttcccgcta cagaaaccat gcccgttatg cgctccaagg
      601 tacttacctg gctgaaagcg cattctgaaa cactggacaa gccggttggt gaaagtgttt
      661 ttgccgccct gcacgcctct tatccgtgca gtgtgaaaaa atgaaagcag caattatcgt
      721 attccccggt accaaccggg agcgcgatat ggccattgcc ctcaagcgcg ttacagggca
      781 ggccccgcgc atgatctggc accatgaaac agatctggga gataccgatc tagtcgtgct
      841 gccaggtggt ttcagctatg gcgattatct gcgctgtggt gccatggcag cgcattctcc
      901 catcatggcg gctatcaaag cctttgccca aaagggtggc catattttgg gtgtgtgcaa
      961 cggcttccag attttaacgg aaacagggct tctgcccggt gctctgttgc gcaacgccaa
     1021 cctgcgcttt t
//
