LOCUS       sequence-135982-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit with N-terminal His6-tag (H6AaPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK620
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             354..371
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(424..442)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      438..485
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      522..977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(609..628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     819..840
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaaggtac gtgtaaccgt catgctgaag
      121 gacggtgttc tggacccgca gggcaaggcc atcagccacg cattgcagac acttggtttt
      181 gaaggcgtgc acgatgtgcg cgtgggtaag gtgattgaac tggatctgcc gggtgccaaa
      241 gccgaggaag cccagaaaaa ggccgatgct atggcgcgtg atctgctagc caacctggtg
      301 attgaagatt actctgtaga ggtggtggaa tgaagcttgc ggccgcactc gagcaccacc
      361 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      421 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      481 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      961 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1021 ttatttttct aaatacattc aaa
//
