LOCUS       sequence-135984-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase large subunit with N-terminal His6-tag (H6AaPurL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK622
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgaa taagcctgta accgttgatg
      121 aatcccttgc ccgtgaattc gggctgacat ctgaggaata cggtcgtgtg ctgtccatta
      181 tgggccgcac ccccagcctg acagagctgg gcgtgttctc tgtgatgtgg tcggaacact
      241 gctcctacaa atcttcccgc aagtggctgc gcacgctgcc aaccaaggcg ccgtgggtta
      301 ttcatggccc cggtgaaaat gctggtgtgg tggatattgg gcagggctat gccgccatct
      361 tcaagatgga aagccataac cacccatcct ttatcgaacc ctatcagggt gcggccacgg
      421 gtgtgggggg cattctgcgc gatgtgttca ccatgggcgc acgccccatt gccaacttga
      481 acgccctgcg ttttggcagc cccgaaaacc ccgttacccg ccgcgtgatt gatggcgtgg
      541 tgcgtggtat tggcggctat ggcaactgtg ttggcgtgcc caccgtaggc ggggaaatca
      601 acttccaccc ggcctatgat ggcaacccgc tggtaaacgc catgacggtt ggtttggccc
      661 gtaaggaccg tatcttcctt tcggctgcgg caggtgttgg caacccggtt gtttacgtgg
      721 gctcccgcac agggcgtgat ggcattcatg gtgccaccat gtcttccgcc gagtttgatg
      781 aagatgccct ttccaagcgc cccacggtac aggtgggtga cccctttatt gaaaagcttc
      841 tgattgaagc ctgtctggaa ctgatggcaa cagatgccat tatcgccatt caggacatgg
      901 gcgctgcggg tctgacatct tcttccgtgg aaatggcagg caagggcggt gtgggcattg
      961 agcttgatct ggatgccgtg ccccagcgtg agccgaacat gacggcttat gaaatgatgc
     1021 tgtctgaaag ccagga
//
