LOCUS       sequence-135989-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRed for monosynaptic circuit mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-dsRed
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..687
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(140..158)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(190..210)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     408..427
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     602..625
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aaagccacca tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc
       61 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      121 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
      181 tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc
      241 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      301 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggctcc
      361 ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag
      421 aagaagacta tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
      481 aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
      541 aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc
      601 aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcgcc
      661 gagggccgcc accacctgtt cctgtag
//
