LOCUS       sequence-135993-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase small subunit (AaPurS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK544
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    155..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(180..198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    558..582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(583..601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        647..724
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(744..763)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgaggg
       61 taccgacgtc agcgatcgcg tggccggccg atatccaatt gagatctgcc atatgtatat
      121 ctccttctta tacttaacta atatactaag atggggaatt gttatccgct cacaattccc
      181 ctatagtgag tcgtattaat ttcgattatg cggccgtgta caatacgatt actttctgtt
      241 cgacttaagc attatgcggc cgcaagcttc attccaccac ctctacagag taatcttcaa
      301 tcaccaggtt ggctagcaga tcacgcgcca tagcatcggc ctttttctgg gcttcctcgg
      361 ctttggcacc cggcagatcc agttcaatca ccttacccac gcgcacatcg tgcacgcctt
      421 caaaaccaag tgtctgcaat gcgtggctga tggccttgcc ctgcgggtcc agaacaccgt
      481 ccttcagcat gacggttaca cgtaccttcg ccatggtata tctccttatt aaagttaaac
      541 aaaattattt ctacagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt
      601 aatttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg
      661 gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
      721 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
      781 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
      841 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
      901 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
      961 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tg
//
