LOCUS       sequence-135999-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical protein in purSyQL 
            cluster (AaOrfY).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK567
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    158..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    691..715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        780..857
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(877..896)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtaccgac gtcagcgatc gcgtggccgg ccgatatcca attgagatct gccatatgta
      121 tatctccttc ttatacttaa ctaatatact aagatgggga attgttatcc gctcacaatt
      181 cccctatagt gagtcgtatt aatttcgatt atgcggccgt gtacaatacg attactttct
      241 gttcgactta agcattatgc ggccgcaagc tttcattttt tcacactgca cggataagag
      301 gcgtgcaggg cggcaaaaac actttcacca accggcttgt ccagtgtttc agaatgcgct
      361 ttcagccagg taagtacctt ggagcgcata acgggcatgg tttctgtagc gggaacacaa
      421 aaaccagcag gcgcagaagg atccccctcg ttccgatcat tgattttgga aagagagacc
      481 ccatccgtca ggccgccaat ataggcatcg caaatgccag cactagtggc gcgcgtacaa
      541 aggcgggcaa agttacctgc cttcatgggc gcaatgcgct gggcatgggc caagcccggt
      601 gccgcgcatg ccaatgccag cgctataaga agggtgcgct ttgccatggt atatctcctt
      661 attaaagtta aacaaaatta tttctacagg ggaattgtta tccgctcaca attcccctat
      721 agtgagtcgt attaatttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg
      781 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
      841 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
      901 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
      961 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
     1021 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
     1081 cgccgtcgca aattg
//
