LOCUS       sequence-136000-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of dmBACCS2VL, a modified Drosophila 
            melanogaster blue light-activated Ca2+ channel switch, along with 
            dOrai and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-18"; volume: "6:8021. doi"; pages: " 
            10.1038/ncomms9021"
COMMENT     Sequence Label: dmBACCS2VL-IRES-dOrai-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcctcga ggccaccatg ggaccggtcg gaggaagcgg
       61 tggatctttg gctactacac ttgaacgtat tgagaagaac tttctcatta ctgacccaag
      121 attgccagat aatcccatta tattcgcgtc cgatagtttc ttgcagttga cagaatatag
      181 ccgtgaagaa attttgggaa gaaactgcag gtttctacaa ggtcctgaaa ctgatcgcgc
      241 gacagtgaga aaaattagag atgccataga taaccaaaca gaggtcactg ttcagctgat
      301 taattataca aagagtggta aaaagttctg gaacctcttt cacttgcagc ctatgcgaga
      361 tcagaaggga gatgtccagt actttattgg ggttcagttg gatggaactg agcatgtccg
      421 agatgctgcc gagagagagg gagtcatgct gattaagaaa actgcagaaa atctgcaatc
      481 atggcttcaa tacacatatg aactagaaag taagaatcat cagaagaagc gcacgtcggc
      541 tgagaagcag ctacagtcgg ccagagaggc ttgtgagaaa ttgcgtaaga aacggtcaag
      601 tttggtgggt gcgttcgttt ccacgcacgg aaagagtatt gatgatgtgg atcggtcgat
      661 tgttgaggca cggaatgccc tcggagatgt aacaaacgag ctgcaagaac gactgcatcg
      721 ctggaagcaa atcgagacgt gccttggctt aaacattgtg aacaacaatg gtctgcccgg
      781 aggatccgga ggaagtggag gaggtatcct gcagtctcgg ggtggcagtg gtggttcagg
      841 cggttctgga tctttggcta ctacacttga acgtattgag aagaactttc tcattactga
      901 cccaagattg ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga
      961 atatagccgt gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc ctgaa
//
