LOCUS       sequence-136001-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of dmBACCS2VL, a modified Drosophila 
            melanogaster blue light-activated Ca2+ channel switch, along with 
            dOrai and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-18"; volume: "6:8021. doi"; pages: " 
            10.1038/ncomms9021"
COMMENT     Sequence Label: dmBACCS2VL-IRES-dOrai-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..779)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(347..366)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     616..634
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttagat cccgggcccg cggtaccgtc
       61 gaccgcggtt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
      121 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      181 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      241 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      301 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
      361 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      421 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      481 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      541 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
      601 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      661 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
      721 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccata
      781 ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcgggg
//
