LOCUS       sequence-136003-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hBACCS2, a human blue light-activated Ca2+ 
            channel switch, and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata 
            T
  TITLE     Light generation of intracellular Ca(2+) signals by a genetically 
            encoded protein BACCS.
  JOURNAL   Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
  PUBMED    26282514
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-08-18"; volume: "6:8021. doi"; pages: " 
            10.1038/ncomms9021"
COMMENT     Sequence Label: hBACCS2-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(79..789)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(357..376)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     626..644
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(951..970)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttagat cccgggcccg
       61 cggtaccgtc gaccgcggtt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      121 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
      181 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      241 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
      301 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
      361 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      421 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
      481 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
      541 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
      601 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
      661 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
      721 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
      781 gctcaccata ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc
      841 ctagacgttt ttttaacctc gactaaacac atgtaaagca tgtgcaccga ggccccagat
      901 cagatcccat acaatggggt accttctggg catccttcag ccccttgttg aatacgcttg
      961 aggagagcca tttgactctt tccacaacta tccaactcac aacgtggcac tggggttgtg
     1021 ccgcctttgc aggtgtatct tatacacgtg gcttttggcc gcagaggcac ctgttgccag
     1081 gtggggggtt ccgctgcctg caaag
//
