LOCUS       sequence-136007-        1632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APEX2 fused to Venus for transsynaptic tracing and the EM 
            analysis of infected neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-APEX2-Venus
FEATURES             Location/Qualifiers
     source          1..1632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..36
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             37..783
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             790..816
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      832..841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(838..840,841..843,844..1554)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(883..904)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1144..1163)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1491..1512
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaagccacca tggactacaa ggatgacgac gataagggaa agtcttaccc aactgtgagt
       61 gctgattacc aggacgccgt tgagaaggcg aagaagaagc tcagaggctt catcgctgag
      121 aagagatgcg ctcctctaat gctccgtttg gcattccact ctgctggaac ctttgacaag
      181 ggcacgaaga ccggtggacc cttcggaacc atcaagcacc ctgccgaact ggctcacagc
      241 gctaacaacg gtcttgacat cgctgttagg cttttggagc cactcaaggc ggagttccct
      301 attttgagct acgccgattt ctaccagttg gctggcgttg ttgccgttga ggtcacgggt
      361 ggacctaagg ttccattcca ccctggaaga gaggacaagc ctgagccacc accagagggt
      421 cgcttgcccg atcccactaa gggttctgac catttgagag atgtgtttgg caaagctatg
      481 gggcttactg accaagatat cgttgctcta tctgggggtc acactattgg agctgcacac
      541 aaggagcgtt ctggatttga gggtccctgg acctctaatc ctcttatttt cgacaactca
      601 tacttcacgg agttgttgag tggtgagaag gaaggtctcc ttcagctacc ttctgacaag
      661 gctcttttgt ctgaccctgt attccgccct ctcgttgaca aatatgcagc ggacgaagat
      721 gccttctttg ctgattacgc tgaggctcac caaaagcttt ccgagcttgg gtttgctgat
      781 gccctgcagc tgcctcccct ggagcgcctg accctggacg gaattccggt cgccaccatg
      841 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      901 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      961 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1021 gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     1081 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1141 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1201 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1261 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1321 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     1381 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1441 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1501 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga
     1561 ctcagatcta agctgaaccc tcctgatgag agtggccccg gctgcatgag ctgcaagtgt
     1621 gtgctctcct ga
//
