LOCUS       sequence-136022-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luminescent and fluorescent dual reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..634)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(118..138)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     615..634
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(635..688)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgacga attctcaggc accgggcttg cgggtcatgc
       61 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
      121 cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct
      181 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
      241 gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
      301 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
      361 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg
      421 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
      481 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
      541 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
      601 tggcgaggcg caccgtgggc ttgtactcgg tcattgggcc aggattctcc tcgacgtcac
      661 cgcatgttag cagacttcct ctgccctctc cactgccgtc gacgcgagat ccggtggagc
      721 cgggtccggc ggtgccgtcc acggcagaat tggacgactg agcgcgggat ctggcgaagg
      781 caatgggggt cttgaaggcg tgctggtact ccacgatgcc cagctcggtg tt
//
