LOCUS       sequence-136023-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luminescent and fluorescent dual reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..525
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             541..597
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 attggatcca ccatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc
       61 ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc
      121 ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc
      181 gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa
      241 aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat
      301 ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat
      361 gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc
      421 aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc
      481 atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgaagct tggaagcgga
      541 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg
      601 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
      661 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
      721 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
      781 gtgatgggct acggcttcta ccacttcggc acctacc
//
