LOCUS       sequence-136025-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc reporter under EF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Nluc
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..528
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(594..614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 attggatcca ccatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc
       61 ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc
      121 ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc
      181 gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa
      241 aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat
      301 ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat
      361 gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc
      421 aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc
      481 atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgtaaga attcgtcgac
      541 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
      601 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      661 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      721 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc a
//
