LOCUS       sequence-136026-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc reporter under EF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-Nluc
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..542)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     intron          567..770
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(590..610)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tccagaggtt gattgtcgac gaattcttac gccagaatgc gttcgcacag ccgccagccg
       61 gtcactccgt tgatggttac tcggaacagc agggagccgt cggggttgat caggcgctcg
      121 tcgataattt tgttgccgtt ccacagggtc cctgttacag tgatcttttt gccgtcgaac
      181 acggcgatgc cttcatacgg ccgtccgaaa tagtcgatca tgttcggcgt aaccccgtcg
      241 attaccagtg tgccatagtg caggatcacc ttaaagtgat gatcatccac agggtacacc
      301 accttaaaaa ttttttcgat ctggcccatt tggtcgccgc tcagaccttc atacgggatg
      361 atgacatgga tgtcgatctt cagcccattt tcaccgctca ggacaatcct ttggatcgga
      421 gttacggaca ccccgagatt ctgaaacaaa ctggacacac ctccctgttc aaggacttgg
      481 tccaggttgt agccggctgt ctgtcgccag tccccaacga aatcttcgag tgtgaagacc
      541 atggtggatc caattcctca cgacacctga aatggaagaa aaaaactttg aaccactgtc
      601 tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac
      661 atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc
      721 gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg
      781 aggtgcctgg acggcgccc
//
