LOCUS       sequence-136028-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express fluorescent and luminescence dual reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..633)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(117..137)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     614..633
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(634..687)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgacgaa ttctcaggca ccgggcttgc gggtcatgca
       61 ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc
      121 gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc
      181 ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg
      241 cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag
      301 gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc
      361 gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc
      421 ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag
      481 ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc
      541 ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt
      601 ggcgaggcgc accgtgggct tgtactcggt cattgggcca ggattctcct cgacgtcacc
      661 gcatgttagc agacttcctc tgccctctcc actgccgtcg acgcgagatc cggtggagcc
      721 gggtccggcg gtgccgtcca cggcagaatt ggacgactga gcgcgggatc tggcgaaggc
      781 aatgggggtc ttgaaggcgt gctggtactc cacgatgccc agctcggtgt tgctgtgcag
      841 ctcctccacg cggcggaagg cgaacatggg gcccccgttc tgcaggatgc tggggtggat
      901 ggcgctcttg aagtgcatgt ggc
//
