LOCUS       sequence-136031-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1S-Nluc
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..549)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     LTR             587..855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgacgaa ttcttacgcc agaatgcgtt cgcacagccg
       61 ccagccggtc actccgttga tggttactcg gaacagcagg gagccgtcgg ggttgatcag
      121 gcgctcgtcg ataattttgt tgccgttcca cagggtccct gttacagtga tctttttgcc
      181 gtcgaacacg gcgatgcctt catacggccg tccgaaatag tcgatcatgt tcggcgtaac
      241 cccgtcgatt accagtgtgc catagtgcag gatcacctta aagtgatgat catccacagg
      301 gtacaccacc ttaaaaattt tttcgatctg gcccatttgg tcgccgctca gaccttcata
      361 cgggatgatg acatggatgt cgatcttcag cccattttca ccgctcagga caatcctttg
      421 gatcggagtt acggacaccc cgagattctg aaacaaactg gacacacctc cctgttcaag
      481 gacttggtcc aggttgtagc cggctgtctg tcgccagtcc ccaacgaaat cttcgagtgt
      541 gaagaccatg gtggatccgt ctagcgtagg cgccggtcac agcttggatc tgtaacggcg
      601 cagaacagaa aacgaaacaa agacgtagag ttgagcaagc agggtcaggc aaagcgtgga
      661 gagccggctg agtctaggta ggctccaagg gagcgccgga caaaggcccg gtctcgacct
      721 gagctttaaa cttacctaga cggcggacgc agttcaggag gcaccacagg cgggaggcgg
      781 cagaacgcga ctcaaccggc gtggatggcg gcctcaggta gggcggcggg cgcgtgaagg
      841 agagatgcga gcccctcgaa gcttcagctg tgttctggcg gcaaacccgt tgcgaa
//
