LOCUS       sequence-136033-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1S-copGFP
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(115..777)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgacga attcggcgcg ccttagcgag atccggtgga
       61 gccgggtccg gcggtgccgt ccacggcaga attggacgac tgagcgcggg atctggcgaa
      121 ggcaatgggg gtcttgaagg cgtgctggta ctccacgatg cccagctcgg tgttgctgtg
      181 cagctcctcc acgcggcgga aggcgaacat ggggcccccg ttctgcagga tgctggggtg
      241 gatggcgctc ttgaagtgca tgtggctgtc caccacgaag ctgtagtagc cgccgtcgcg
      301 caggctgaag gtgcgggcga agctgcccac cagcacgtta tcgcccatgg ggtgcaggtg
      361 ctccacggtg gcgttgctgc ggatgatctt gtcggtgaag atcacgctgt cctcggggaa
      421 gccggtgccc accaccttga agtcgccgat cacgcggccg gcctcgtagc ggtagctgaa
      481 gctcacgtgc agcacgccgc cgtcctcgta cttctcgatg cgggtgttgg tgtagccgcc
      541 gttgttgatg gcgtgcagga aggggttctc gtagccgctg gggtaggtgc cgaagtggta
      601 gaagccgtag cccatcacgt ggctcagcag gtaggggctg aaggtcaggg cgcctttggt
      661 gctcttcatc ttgttggtca tgcggccctg cttgggggtg ccctctccgc cgcccaccag
      721 ctcgaactcc acgccgttca gggtgccggt gatgcggcac tcgatctcca tggcgggcag
      781 gccgctctcg tcgctctcca tggtggatcc gtcta
//
