LOCUS       sequence-136034-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CMV-Nluc
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..594
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(660..680)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
       61 tagagctagg gatccaccat ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag
      121 acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag
      181 aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg
      241 aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag
      301 atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg
      361 cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg
      421 ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg
      481 aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga
      541 gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc gtaagaattc
      601 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
      661 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
      721 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
      781 gagttgtggc
//
