LOCUS       sequence-136035-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CMV-Nluc
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..551)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(615..639)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(636..839)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(665..685)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgacg aattcttacg ccagaatgcg ttcgcacagc
       61 cgccagccgg tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc
      121 aggcgctcgt cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg
      181 ccgtcgaaca cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta
      241 accccgtcga ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca
      301 gggtacacca ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca
      361 tacgggatga tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt
      421 tggatcggag ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca
      481 aggacttggt ccaggttgta gccggctgtc tgtcgccagt ccccaacgaa atcttcgagt
      541 gtgaagacca tggtggatcc ctagctctag aatcttctat ggaggtcaaa acagcgtgga
      601 tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata aacctcccac
      661 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
      721 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
      781 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
      841 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
//
