LOCUS       sequence-136038-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc and copGFP dual reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..535
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             551..607
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgggcctttc gacggatcca ccatggtctt cacactcgaa gatttcgttg gggactggcg
       61 acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt
      121 tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg
      181 gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg
      241 ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat
      301 cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg
      361 acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct
      421 gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt
      481 ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgaagct
      541 tggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc
      601 tggacctatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
      661 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
      721 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcc
//
