LOCUS       sequence-136040-         469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nluc, copGFP dual reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 469)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-CB-Nluc-P2A-copGFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             303..359
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact
       61 atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt
      121 atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg
      181 gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa
      241 ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgaag cttggaagcg
      301 gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta
      361 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc
      421 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaa
//
