LOCUS       sequence-136044-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express EGFP from the 
            tetO promoter under intersectional control by Flp and Cre 
            recombinases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    104..137
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(148..181)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    341..389
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     414..433
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 gtaagaattc cttgggcccc ctcgaaggta cgttgcgtta tttaaatttg gcgcgccaaa
       61 taacttcgta taaagtatcc tatacgaagt tattgcgatt cggataactt cgtataatgt
      121 atgctatacg aagttatcga cgtcgaggaa gttcctatac tttctagaga ataggaactt
      181 ccacggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg
      241 tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta
      301 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc
      361 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
      421 cttccgtgtt tcagttagcc tcccccctac tcaattagct gaggcagaag gacagggaag
      481 ggagcagtgg ttcacgcctg taatcccagc aatttgggag gccaaggtgg gtagatcacc
      541 tgagattagg agttggagac cagcctggcc aatatggtga aaccccgtct ctaccaaaaa
      601 aacaaaaatt agctgagcct ggtcatgcat gcctggaatc ccaacaactc gggagcctga
      661 ggcaggagaa tcgcttgaac ccaggagg
//
