LOCUS       sequence-136049-        2055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry and FlpO for monosynaptic circuit mapping and 
            genetic manipulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
FEATURES             Location/Qualifiers
     source          1..2055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..717
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(155..173)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     423..442
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             727..783
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aaagccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
       61 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      121 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      181 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      241 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      301 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      361 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      421 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      481 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      541 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      601 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      661 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga
      721 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
      781 cctatgagcc agttcgacat cctgtgcaag acccccccca aggtgctggt gcggcagttc
      841 gtggagagat tcgagaggcc cagcggcgag aagatcgcca gctgtgccgc cgagctgacc
      901 tacctgtgct ggatgatcac ccacaacggc accgccatca agagggccac cttcatgagc
      961 tacaacacca tcatcagcaa cagcctgagc ttcgacatcg tgaacaagag cctgcagttc
     1021 aagtacaaga cccagaaggc caccatcctg gaggccagcc tgaagaagct gatccccgcc
     1081 tgggagttca ccatcatccc ttacaacggc cagaagcacc agagcgacat caccgacatc
     1141 gtgtccagcc tgcagctgca gttcgagagc agcgaggagg ccgacaaggg caacagccac
     1201 agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga gcatctggga gatcaccgag
     1261 aagatcctga acagcttcga gtacaccagc aggttcacca agaccaagac cctgtaccag
     1321 ttcctgttcc tggccacatt catcaactgc ggcaggttca gcgacatcaa gaacgtggac
     1381 cccaagagct tcaagctggt gcagaacaag tacctgggcg tgatcattca gtgcctggtg
     1441 accgagacca agacaagcgt gtccaggcac atctactttt tcagcgccag aggcaggatc
     1501 gaccccctgg tgtacctgga cgagttcctg aggaacagcg agcccgtgct gaagagagtg
     1561 aacaggaccg gcaacagcag cagcaacaag caggagtacc agctgctgaa ggacaacctg
     1621 gtgcgcagct acaacaaggc cctgaagaag aacgccccct accccatctt cgctatcaag
     1681 aacggcccta agagccacat cggcaggcac ctgatgacca gctttctgag catgaagggc
     1741 ctgaccgagc tgacaaacgt ggtgggcaac tggagcgaca agagggcctc cgccgtggcc
     1801 aggaccacct acacccacca gatcaccgcc atccccgacc actacttcgc cctggtgtcc
     1861 aggtactacg cctacgaccc catcagcaag gagatgatcg ccctgaagga cgagaccaac
     1921 cccatcgagg agtggcagca catcgagcag ctgaagggca gcgccgaggg cagcatcaga
     1981 taccccgcct ggaacggcat catcagccag gaggtgctgg actacctgag cagctacatc
     2041 aacaggcgga tctga
//
