LOCUS       sequence-136050-        1815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry and Cre for monosynaptic circuit mapping and 
            genetic manipulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-mCherry-P2A-Cre
FEATURES             Location/Qualifiers
     source          1..1815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..717
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(155..173)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     423..442
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             727..783
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             784..1815
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
ORIGIN
        1 aaagccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
       61 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      121 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      181 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      241 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      301 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      361 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      421 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      481 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      541 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      601 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      661 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga
      721 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
      781 cctatgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg
      841 agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag
      901 catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat
      961 aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag
     1021 gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat
     1081 cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg
     1141 atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc
     1201 actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca ggatatacgt
     1261 aatctggcat ttctggggat tgcttataac accctgttac gtatagccga aattgccagg
     1321 atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga
     1381 acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa
     1441 ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt
     1501 tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc
     1561 gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct
     1621 ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg
     1681 gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat
     1741 attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg
     1801 gaagatggcg attaa
//
