LOCUS       sequence-136077-        8829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expression of mOC-STAMP with N162D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue 
            LR, Birnbaum MJ, Odgren PR
  TITLE     Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse 
            Model, Rescue of Cell Fusion, and Transmembrane Topology.
  JOURNAL   PLoS One. 2015 Jun 4;10(6):e0128275. doi: 
            10.1371/journal.pone.0128275. eCollection 2015.
  PUBMED    26042409
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-4"; volume: "10(6):e0128275. doi"; pages: " 
            10.1371/journal.pone.0128275. eCollection 2015"
COMMENT     Sequence Label: pLenti-CMV-mOC-STAMP (N162D)-GFP
FEATURES             Location/Qualifiers
     source          1..8829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..606
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(71..91)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(489..500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(748..781)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             801..981
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1053..1174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1090..1109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1144..1163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1378..1397)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1379..1397)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1407..1424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1407..1423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1416..1438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1568..2023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1655..1674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1865..1886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2052..2156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2157..2225,2226..3017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2375..2394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3188..3776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3677..3696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3930..3947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4064..4085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4100..4105,4106..4123,4124..4130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4138..4154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4143..4165
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4162..4178
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4162..4178
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4197..4217
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4199..4217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             4473..4653
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4697..4822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5315..5548
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5732..5776
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6037..6154
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6177..6480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6481..6684
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6635..6655
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6681..6705
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(6773..6775,6776..6778,6779..7492)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6773..6775,6776..6778,6779..7489)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6818..6839)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7079..7098)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7426..7447
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7501..8073
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7524..8075
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7668..7685)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7895..7914
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8101..8700
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(8101..8120)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8597..8617
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctagctggcc agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
       61 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      121 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
      181 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
      241 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
      301 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
      361 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
      421 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
      481 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
      541 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
      601 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
      661 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
      721 acaagatggg atcaattcac catgggaata acttcgtata gcatacatta tacgaagtta
      781 tgctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      961 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     1021 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     1081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     1201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     1261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     1321 tggaggatcg aattcctgca gccccctagg cttttgcgtc gagacgtacc caattcgccc
     1381 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     1441 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1501 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1561 tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1621 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1681 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1741 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1801 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1861 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1921 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1981 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccaggt ggcacttttc
     2041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3301 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3961 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4021 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4081 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4141 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     4201 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     4261 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     4321 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     4381 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     4441 tgtatttaag tgcctagctc gatacaataa acgggtctct ctggttagac cagatctgag
     4501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4621 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acctgaaagc
     4681 gaaagggaaa ccagagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     4741 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     4801 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     4861 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     4921 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     4981 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     5041 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     5101 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     5161 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     5221 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     5281 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     5341 gagcagcagg aagcactatg ggcgcagcct caatgacgct gacggtacag gccagacaat
     5401 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     5461 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     5521 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     5581 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagattggaa
     5641 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5701 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5761 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5821 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5881 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5941 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     6001 atccattcga ttagtgaacg gatctcgacg gttaactttt aaaagaaaag gggggattgg
     6061 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     6121 attacaaaaa caaattacaa aaattcaaaa ttttatcgat aagcttggga gttccgcgtt
     6181 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6241 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6301 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6361 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6421 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6481 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     6541 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6601 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     6661 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     6721 ccacgctgtt ttgacctcca tagaagacac cgactctaga ggatccccgc ggatggtgag
     6781 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6841 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6901 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6961 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7021 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7081 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7141 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7201 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7261 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7321 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7381 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7441 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagaattccg
     7501 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7561 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7621 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7681 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7741 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7801 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7861 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7921 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7981 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     8041 gacgtggttt tcctttgaaa aacacgatga taatatgggg gctagagcgg ccagcttacc
     8101 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     8161 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     8221 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     8281 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     8341 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     8401 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     8461 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     8521 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     8581 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     8641 gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga
     8701 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata
     8761 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     8821 tttggcaag
//
